Abstract
A Pseudomonas aeruginosa strain (UCBPP-PA14) is infectious both in an Arabidopsis thaliana leaf infiltration model and in a mouse full-thickness skin burn model. UCBPP-PA14 exhibits ecotype specificity for Arabidopsis, causing a range of symptoms from none to severe in four different ecotypes. In the mouse model, UCBPP-PA14 is as lethal as other well-studied P. aeruginosa strains. Mutations in the UCBPP-PA14 toxA, plcS, and gacA genes resulted in a significant reduction in pathogenicity in both hosts, indicating that these genes encode virulence factors required for the full expression of pathogenicity in both plants and animals.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ADP Ribose Transferases*
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Animals
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Arabidopsis / microbiology*
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Bacterial Proteins / genetics
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Bacterial Toxins*
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Base Sequence
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Burns / complications
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Exotoxins / genetics
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Male
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Mice
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Molecular Sequence Data
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Mutation
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Phospholipases / genetics
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Plant Diseases / microbiology*
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Pseudomonas Infections / microbiology*
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Pseudomonas aeruginosa / genetics
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Pseudomonas aeruginosa / growth & development
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Pseudomonas aeruginosa / pathogenicity*
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Pseudomonas aeruginosa Exotoxin A
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Virulence / genetics
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Virulence Factors*
Substances
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Bacterial Proteins
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Bacterial Toxins
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Exotoxins
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GacA protein, Bacteria
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Virulence Factors
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ADP Ribose Transferases
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Phospholipases