Two independent pathways of expression lead to self-assembly of the rabbit hemorrhagic disease virus capsid protein

J Virol. 1995 Sep;69(9):5812-5. doi: 10.1128/JVI.69.9.5812-5815.1995.

Abstract

The rabbit hemorrhagic disease virus capsid protein was expressed in insect cells either as an individual protein species, from a mRNA analogous to the viral subgenomic RNA, or as part of a polyprotein that included the viral 3C-like protease and the RNA polymerase. Both pathways of expression led to the assembly of viruslike particles morphologically and antigenically similar to purified virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Baculoviridae
  • Base Sequence
  • Capsid / analysis
  • Capsid / biosynthesis*
  • Cell Line
  • DNA Primers
  • DNA Probes
  • Gene Expression*
  • Genetic Vectors
  • Hemorrhagic Disease Virus, Rabbit / genetics
  • Hemorrhagic Disease Virus, Rabbit / metabolism*
  • Hemorrhagic Disease Virus, Rabbit / ultrastructure
  • Insecta
  • Microscopy, Electron
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Recombinant Proteins / analysis
  • Recombinant Proteins / biosynthesis

Substances

  • DNA Primers
  • DNA Probes
  • Recombinant Proteins