Sequence variation in the 5', putative promoter of Sry and its possible relevance to the C57BL/6J-YDOM sex reversal

Biochem Biophys Res Commun. 1995 Mar 17;208(2):624-8. doi: 10.1006/bbrc.1995.1384.

Abstract

Sry is the testes determining factor gene located on the Y chromosome. We sought sequence variation in potential promoter regions that might explain the gradiation in sex reversal seen when different domesticus strains are bred to C57BL/6J females since sequence variation previously found in the coding region is not sufficient to explain it. Dideoxy sequencing identified a 10 bp deletion in the 5' flanking region limited to YDOM chromosomes of weak effect. A PCR strategy to search for deletions in this region disclosed another deletion which was shown to be different by sequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA Primers / chemistry
  • DNA-Binding Proteins / genetics*
  • Female
  • Gene Expression Regulation, Developmental
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Nuclear Proteins*
  • Promoter Regions, Genetic*
  • RNA, Messenger / genetics
  • Sequence Deletion
  • Sex Determination Analysis*
  • Sex-Determining Region Y Protein
  • Transcription Factors*

Substances

  • DNA Primers
  • DNA-Binding Proteins
  • Nuclear Proteins
  • RNA, Messenger
  • Sex-Determining Region Y Protein
  • Sry protein, mouse
  • Transcription Factors

Associated data

  • GENBANK/S76890