A new sensitive sandwich enzyme-linked immunosorbent assay to detect galactofuran in patients with invasive aspergillosis

J Clin Microbiol. 1995 Feb;33(2):497-500. doi: 10.1128/jcm.33.2.497-500.1995.

Abstract

A double-direct sandwich enzyme-linked immunosorbent assay that uses a rat anti-galactomannan monoclonal antibody as the acceptor and detector antibody was designed. This immunoassay, which detects less than 1 ng of galactomannan per ml, was assessed in a retrospective study with samples from patients with invasive aspergillosis. Serum is more appropriate than urine for use in the search for circulating galactomannan. Antigenemia does not have a transient character. Galactomannan can be detected at least 39 days before the death of the patients.

MeSH terms

  • Adolescent
  • Adult
  • Antigens, Fungal / blood*
  • Antigens, Fungal / urine
  • Aspergillosis / diagnosis
  • Aspergillosis / immunology
  • Aspergillosis / microbiology*
  • Aspergillus / immunology*
  • Aspergillus / isolation & purification
  • Child, Preschool
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Enzyme-Linked Immunosorbent Assay / statistics & numerical data
  • Evaluation Studies as Topic
  • False Positive Reactions
  • Female
  • Galactose / analogs & derivatives
  • Humans
  • Male
  • Mannans / blood*
  • Mannans / immunology
  • Mannans / urine
  • Middle Aged
  • Opportunistic Infections / diagnosis
  • Opportunistic Infections / immunology
  • Opportunistic Infections / microbiology
  • Sensitivity and Specificity

Substances

  • Antigens, Fungal
  • Mannans
  • galactomannan
  • Galactose