Abstract
A double-direct sandwich enzyme-linked immunosorbent assay that uses a rat anti-galactomannan monoclonal antibody as the acceptor and detector antibody was designed. This immunoassay, which detects less than 1 ng of galactomannan per ml, was assessed in a retrospective study with samples from patients with invasive aspergillosis. Serum is more appropriate than urine for use in the search for circulating galactomannan. Antigenemia does not have a transient character. Galactomannan can be detected at least 39 days before the death of the patients.
MeSH terms
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Adolescent
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Adult
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Antigens, Fungal / blood*
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Antigens, Fungal / urine
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Aspergillosis / diagnosis
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Aspergillosis / immunology
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Aspergillosis / microbiology*
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Aspergillus / immunology*
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Aspergillus / isolation & purification
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Child, Preschool
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Enzyme-Linked Immunosorbent Assay / methods*
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Enzyme-Linked Immunosorbent Assay / statistics & numerical data
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Evaluation Studies as Topic
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False Positive Reactions
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Female
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Galactose / analogs & derivatives
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Humans
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Male
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Mannans / blood*
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Mannans / immunology
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Mannans / urine
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Middle Aged
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Opportunistic Infections / diagnosis
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Opportunistic Infections / immunology
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Opportunistic Infections / microbiology
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Sensitivity and Specificity
Substances
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Antigens, Fungal
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Mannans
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galactomannan
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Galactose