Neurotrophin-3 (NT-3) levels in the developing rat nervous system and in human samples

Clin Chim Acta. 1994 Jun;227(1-2):23-36. doi: 10.1016/0009-8981(94)90132-5.

Abstract

We developed an enzyme immunoassay (EIA) system for neurotrophin-3 (NT-3), based on a biotin-streptavidin detection system capable of measuring concentrations as low as 0.5 pg/ml with high reproducibility. We measured NT-3 levels in the developing rat nervous system as well as in normal subjects and patients with Alzheimer's disease (AD). The developmental change of NT-3 level in rat brain and peripheral tissues coincided with the change in NT-3 mRNA level reported before. We could not find any differences in NT-3 level in serum, cerebrospinal fluid, or brain (hippocampus and parietal cortex) obtained from controls and patients with AD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Alzheimer Disease / metabolism*
  • Animals
  • Brain Chemistry
  • Cells, Cultured
  • Central Nervous System / chemistry*
  • Central Nervous System / growth & development*
  • Humans
  • Immunoenzyme Techniques*
  • Mice
  • Mice, Inbred ICR
  • Middle Aged
  • Nerve Growth Factors / analysis*
  • Neurotrophin 3
  • Rats
  • Viscera / chemistry

Substances

  • Nerve Growth Factors
  • Neurotrophin 3