Abstract
Three human neuroblastoma cell lines were examined to determine the effect of recombinant gamma-interferon (IFN-gamma) treatment on the expression of trk proto-oncogene. Increased levels of trk proto-oncogene mRNA were observed in two neuroblastoma cell lines (KP-N-RT and KP-N-SI(FA)) after IFN-gamma treatment. The levels of trk mRNA increased with growth inhibition and morphological change in a time- and dose-dependent manner. The decreased level of N-myc mRNA after IFN-gamma treatment in KP-N-RT was inversely correlated with trk mRNA. Our results suggest that IFN-gamma can modulate the signal transduction of nerve growth factor in human neuroblastoma cells.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Blotting, Northern
-
Dose-Response Relationship, Drug
-
Gene Expression Regulation, Neoplastic / drug effects*
-
Genes, myc / drug effects
-
Humans
-
Interferon-gamma / pharmacology*
-
Nerve Growth Factors / metabolism
-
Neuroblastoma / chemistry
-
Neuroblastoma / genetics*
-
Oncogene Proteins / biosynthesis
-
Oncogene Proteins / genetics*
-
Proto-Oncogene Mas
-
Proto-Oncogenes / drug effects*
-
RNA, Messenger / metabolism
-
RNA, Neoplasm / metabolism
-
Signal Transduction / drug effects
-
Tumor Cells, Cultured / drug effects
Substances
-
MAS1 protein, human
-
Nerve Growth Factors
-
Oncogene Proteins
-
Proto-Oncogene Mas
-
RNA, Messenger
-
RNA, Neoplasm
-
oncogene protein trk
-
Interferon-gamma