Selective advantage of normal erythrocyte production after bone marrow transplantation of alpha-thalassemic mice

Exp Hematol. 1994 May;22(5):441-6.

Abstract

Anemia resulting from alpha-thalassemia in mice was corrected by transplantation of normal bone marrow cells following sublethal total body irradiation, resulting in partial hematopoietic chimerism with a preponderance of normal peripheral blood red cells. Peripheral blood red cell chimerism in recipients of graded numbers of bone marrow cells from sex-mismatched donors, determined by cytometric analysis, was directly compared with immature hematopoietic cell (CFU-S) chimerism and peripheral blood white cell chimerism. The latter two were assessed by fluorescent in situ hybridization with a murine Y-chromosome-specific probe. Peripheral blood white cell chimerism consistently corresponded with immature hematopoietic cell chimerism, emphasizing the selective advantage of normal red cell production in partially chimeric alpha-thalassemic mice.

MeSH terms

  • Animals
  • Bone Marrow / pathology
  • Bone Marrow Transplantation*
  • Chimera
  • Erythrocytes / cytology*
  • Erythrocytes / physiology
  • Erythropoiesis / physiology
  • Female
  • In Situ Hybridization
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Whole-Body Irradiation
  • alpha-Thalassemia / pathology
  • alpha-Thalassemia / therapy*