Loss of NADPH-diaphorase containing neurones after reversible focal ischaemia in rats delayed by L-NAME

Br J Pharmacol. 1995 Nov;116(5):2344-5. doi: 10.1111/j.1476-5381.1995.tb15076.x.

Abstract

In the present study, NADPH-diaphorase histochemistry was used to assess the temporal evolution of the number of nitric oxide (NO)-synthase containing neurones after reversible focal cerebral ischaemia in rats. The number of NADPH-diaphorase containing neurones was reduced by 50% and 90% respectively 6 and 24 h after ischaemia. L-NAME, a NO-synthase inhibitor, prevented the loss of NADPH-diaphorase containing neurones observed 6 h after ischemia but not 24 h after ischaemia, suggesting that in the early phase, nitric oxide is involved in this phenomenon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arginine / analogs & derivatives*
  • Arginine / pharmacology
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / enzymology
  • Cerebral Cortex / pathology
  • Enzyme Inhibitors / pharmacology*
  • Ischemic Attack, Transient / enzymology*
  • Ischemic Attack, Transient / pathology
  • Male
  • NADPH Dehydrogenase / metabolism*
  • NG-Nitroarginine Methyl Ester
  • Neostriatum / drug effects
  • Neostriatum / enzymology
  • Neostriatum / pathology
  • Neurons / drug effects
  • Neurons / enzymology*
  • Nitric Oxide Synthase / antagonists & inhibitors*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Enzyme Inhibitors
  • Arginine
  • Nitric Oxide Synthase
  • NADPH Dehydrogenase
  • NG-Nitroarginine Methyl Ester