Identification of chromosome 21 materials using the whole chromosome 21 specific library

Southeast Asian J Trop Med Public Health. 1995:26 Suppl 1:92-5.

Abstract

The chromosome in situ suppression hybridization or chromosome painting technic was applied to confirm and eliminate the markers involving chromosome 21 segments using a chromosome 21 DNA library. The library ATCCLL21SNO2 was amplified, directly biotinylated using the polymerase chain reaction. The results demonstrated a translocation of chromosome 21 material on chromosome 2 and X and eliminate the origin of the marker. Thus, the technique provides an important tool to complement the conventional G-banding technic.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abnormalities, Multiple / genetics*
  • Adult
  • Chromosomes, Human, Pair 2
  • Chromosomes, Human, Pair 21*
  • Down Syndrome / diagnosis
  • Down Syndrome / genetics*
  • Female
  • Gene Library
  • Genetic Carrier Screening*
  • Genetic Markers
  • Humans
  • Infant, Newborn
  • Intellectual Disability / genetics*
  • Metaphase
  • Translocation, Genetic
  • X Chromosome

Substances

  • Genetic Markers