Characterisation of seven human ovarian tumour cell lines

Br J Cancer. 1996 Sep;74(5):722-7. doi: 10.1038/bjc.1996.428.

Abstract

Characteristics of a panel of seven human ovarian tumour cell lines are presented. Positive staining with HMFG2 and ultrastructural identification of desmosomes confirmed the epithelial nature of the cell lines. The lines showed wide variations in ploidy, doubling times and clonogenicity in soft agar. Both vimentin and keratin were equally expressed in five lines, one line showed strong preferential expression of keratin and one line showed preferential expression of vimentin. Karyotypic changes associated with ovarian cancer were identified in all the lines. Four of the seven cell lines showed loss of chromosome material distal to 11p13-15. These cell lines offer considerable potential for research into the biology and genetics of ovarian cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Antigens, Neoplasm / biosynthesis
  • Biomarkers, Tumor / analysis*
  • CA-125 Antigen / biosynthesis
  • Clone Cells / physiology
  • Cytogenetics
  • Desmosomes / ultrastructure
  • Endoplasmic Reticulum, Rough / ultrastructure
  • Female
  • Humans
  • Immunohistochemistry
  • Karyotyping
  • Keratins / biosynthesis
  • Middle Aged
  • Mucin-1 / biosynthesis
  • Ovarian Neoplasms / chemistry*
  • Ovarian Neoplasms / genetics
  • Ovarian Neoplasms / pathology
  • Tumor Cells, Cultured / chemistry*
  • Tumor Cells, Cultured / pathology
  • Vimentin / biosynthesis

Substances

  • Antigens, Neoplasm
  • Biomarkers, Tumor
  • CA-125 Antigen
  • Mucin-1
  • Vimentin
  • Keratins