Improved ligation-anchored PCR strategy for identification of 5' ends of transcripts

Biotechniques. 1996 Jul;21(1):34-6, 38. doi: 10.2144/96211bm06.
No abstract available

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing
  • Animals
  • Bacteriophage T4 / enzymology
  • Biotin
  • DNA Primers
  • DNA, Complementary / biosynthesis
  • DNA, Complementary / metabolism
  • Exons
  • Humans
  • Mice
  • Nuclear Proteins*
  • Polymerase Chain Reaction / methods*
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins c-mdm2
  • RNA Ligase (ATP) / metabolism
  • RNA, Messenger / analysis*
  • Thiolester Hydrolases / genetics
  • Ubiquitin Thiolesterase

Substances

  • DNA Primers
  • DNA, Complementary
  • Nuclear Proteins
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Biotin
  • MDM2 protein, human
  • Mdm2 protein, mouse
  • Proto-Oncogene Proteins c-mdm2
  • Thiolester Hydrolases
  • Ubiquitin Thiolesterase
  • RNA Ligase (ATP)