The authors developed a new apparatus for extracting nitrogen or other inert gases from blood by flushing (sparging) the specimen with another gas. To investigate the utility of the new methodology, the apparatus was used in conjunction with a mass spectrometer to measure the blood N2 content of healthy normobaric, non-smoking, adult volunteers; the mean was found to be 11.7 microliters/ml +/- 0.9 microliter. This compares closely with values cited in the literature. The within-subject variation for repeat samples taken several weeks apart was significantly (P < 0.003) less than the variation between different subjects, suggesting that there may be true differences in N2 content between different individuals. These data must be considered preliminary, a larger study is needed to investigate population differences in detail. The advantages of the new method are discussed.