On the mechanism of RecA-mediated repair of double-strand breaks: no role for four-strand DNA pairing intermediates

Mol Cell. 1998 Jan;1(2):309-17. doi: 10.1016/s1097-2765(00)80031-3.

Abstract

RecA protein will bind to a gapped duplex DNA molecule and promote a DNA strand exchange with a second homologous linear duplex. A double-strand break in the second duplex is efficiently bypassed in the course of these reactions. We demonstrate that the bypass of double-strand breaks is not explained by a mechanism involving homologous interactions between two duplex DNA molecules, but instead requires the ATP-mediated generation of DNA torsional stress brought about by the action of RecA. The results suggest new pathways for the repair of double-strand breaks and underline the need for new paradigms to explain the alignment of homologous DNAs during genetic recombination.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • DNA / metabolism*
  • DNA Repair / physiology*
  • DNA, Bacterial / genetics*
  • DNA, Single-Stranded / metabolism
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Hydrolysis
  • Rec A Recombinases / metabolism*

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • DNA, Single-Stranded
  • Adenosine Triphosphate
  • DNA
  • Rec A Recombinases