Prairie voles (Microtus ochrogaster) are monogamous New World rodents which show geographic variation in social behavior. In this study, parameters of female reproduction which might be related to mating system were compared in prairie voles from eastern Kansas (KAN) versus central Illinois (ILL). KAN females showed a more rapid onset of natural estrus following exposure to a male and were more likely to respond to injections of a low dose (0.5 microg) of exogenous estradiol benzoate than ILL females. Neither mating duration nor pregnancy success after mate removal differed in KAN versus ILL females. These results suggest that ILL voles are less sensitive than KAN voles to the estrus-inducing effects of either endogenous or exogenous estrogen, supporting the hypothesis that variations in reproductive strategy occur among geographically discrete populations of prairie voles.