Detection of Aspergillus galactomannan: comparison of an enzyme-linked immunoassay and a europium-linked time-resolved fluoroimmunoassay

J Clin Microbiol. 1998 Oct;36(10):3079-80. doi: 10.1128/JCM.36.10.3079-3080.1998.

Abstract

With a view to improving the sensitivity of serological detection of Aspergillus galactomannan (GM), a europium-linked time-resolved fluoroimmunoassay was developed. This method was compared to an enzyme-linked immunosorbent assay using a peroxidase-conjugated detector antibody. No increase in the sensitivity of the detection of GM standards was seen with the europium-based fluoroimmunoassay.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Fungal / analysis*
  • Aspergillosis / diagnosis*
  • Aspergillus / isolation & purification*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Europium
  • Fluorescent Antibody Technique
  • Galactose / analogs & derivatives
  • Humans
  • Mannans / analysis*
  • Sensitivity and Specificity

Substances

  • Antigens, Fungal
  • Mannans
  • galactomannan
  • Europium
  • Galactose